A review of the scientific projects supported by Biohellenika S.A.
Α REVIEW OF THE SCIENTIFIC PROJECTS SUPPORTED BY BIOHELLENIKA S.A.
Biohellenika, an AABB (American Association of Blood Banks) accredited company supports a very strong R&D Department. Highly educated scientists make a remarkable effort in order to improve existed protocols or to launch new products into the market. Eleven papers have been published in distinguished international journals, five patents have been granted and four clinical trials have been supported by this Department. Also many awards are granted to Biohellenika for the pioneer work that has provided. Three PhD theses were supported by Biohellenika and five more are in progress.
AWARDS
- The 1st Award was granted in April 2007 by the Medical Society of Thessaloniki during the 22nd North Greece Medical Conference, concerning the in vitro transformation of progenitor stem cells from the bone marrow to cells of the myocardium, for their future use in patients with myocardial infarction.
- The 2nd Award was granted in May 2007 by the Athens Medical Society during the 33rd Pan-Hellenic Medical Conference, for Biohellenika’s specialized technique of isolation of stem cells with multipotent properties from the placenta drainage.
- The 3rd Award was granted in December 2007 by the Pan-Hellenic Transplantation Society during their 15th Pan-Hellenic Conference, for Biohellenika’s research in the attainment of an additional number of haematopoietic stem cells after draining of the placenta. The adequate number is gained for the treatment of adult patients and especially those with a large body mass.
- The 1st Award from Kerameos foundation in November 2010 by the Cardiovascular Society in the frame of 8th Congress on Cardiovascular Surgery for the combined supporting of left ventricular with bone marrow stem cells and device.
- Award in March 2011 in the frame of 26th Northern Greece Medical Congress for in vitro differentiation of umbilical cord multypotent stem cells to neural cells.
- Biohellenika was awarded in the frame of the 27th Northwestern Medical Congress Thessaloniki 2012 for “Methods of pluripotent stem cells separation and characterization from cord blood, placenta, umbilical cord and adipose tissue".
PUBLICATIONS
- N. Tsagias, K. Kouzi-Koliakos, V. Karagiannis, D.H. Alamdari, G.Koliakos
Time and temperature before processing influence the recovery of umbilical cord blood hematopoietic progenitors.
Transfusion. 2007 Aug;47(8):1550-2.
2. G. Koliakos, D. Alamdari, N. Tsagias, K. Kouzi-Koliakos, E. Michaloudi, V. Karagiannis
A novel high-yield volume-reduction method for the cryopreservation of UC blood units.
Cytotherapy. 2007 Oct 4;:1-6
3. N. Tsagias, K. Kouzi-Koliakos, I. Koliakos, E. Kostidou, V. Karagiannis, A. Daniilidis and G. Koliakos
Addition of Adipose-Derived Stem Cells in Cord Blood Cultures Stimulates Their Pluripotent Differentiation
Transplantation Proceedings 2009, Vol: 41, issue 10:4340-4344
4. N.Tsagias, Iro Koliakos, M. Lappa, V. Karagiannis, and G. G. Koliakos.
Placenta perfusion has hematopoietic and mesenchymal progenitor stem cell potential.
Transfusion 2011. Ω 51, p: 976.
5. Anastasiadis K, Antonitsis P, Argiriadou H, Koliakos G, Doumas A, Khayat A, Papakonstantinou C, Westaby S. Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion. J Transl Med. 2011 Jan 19;9:12
6. N. Tsagias, I. Koliakos, V. Karagiannis, M. Eleftheriadou, G.G. Koliakos.
Isolation of mesenchymal stem cells using the total length of umbilical cord for transplantation purposes. Transfusion Medicine 2011 Aug;21(4):253-61.
7. H. Ravari, D. Hamidi-Almadari, M. Salimifar, S. Bonakdaran, MR. Parizadeh, G.Koliakos Treatment of no-healing woods with autologous bone marrow cells, platelets, fibrin glue and collagen matrix. Cytotherapy. 2011 Jul;13(6):705-11.
8. Tzouvelekis, G. Koliakos, P. Ntolios, I. Baira, E. Bouros, A. Oikonomou, A. Zissimopoulos, G. Kolios, D. Kakagia, V. Paspaliaris, I. Kotsianidis, M. Froudarakis, D. Bouros Stem cell therapy for idiopathic pulmonary fibrosis: A protocol proposal. J of Translational Medicine 2011: 9;182. J Transl Med. 2011 Oct 21;9:182.
9. S. Petrakis, Tamas Raskó 2, Lajos Mátés 3, Zoltan Ivics 4, Zsuzsanna Izsvák Kokkona Kouzi-Koliakou 1, 5 and George Koliakos. Genetic modification of human embryonic kidney and adipose-derived stromal cells using a Gateway compatible transposon vector. Biotechnology Journal 2012 Jul;7(7):891-7.
10. K. Kodonas, C. Gogos, S. Papadimitriou, K. Kouzi-Koliakou, D.Tziafas. Experimental Formation of Dentin-like Structure in the Root Canal Implant Model Using Cryopreserved Swine Dental Pulp Progenitor Cells. J Endod. 2012 Jul;38(7):913-9. Epub 2012 Mar 28.
11. K. Anastasiadis, P. Antonitsis, A. Doumas, G. Koliakos, H. Argiriadou, C. Vaitsopoulou, P. Tossios, C. Papakonstantinou, S. Westaby Stem cell transplantation combined with long-term mechanical circulatory support enhances myocardial viability in end-stage ischemic cardiomyopathy.Inter J Cardiology 2012 Mar 22;155(3):e51-3.
12. V. Karathanasis, S.Petrakis, K.Topouridou, E. Koliakou, G. Koliakos , E. Demiri Intradermal injection of GFP-producing adipose stromal cellSpromotes survival of random-pattern skin flaps in rats. DOI 10.1007/s00238-013-0810-y, 2013
13. J Transl Med. 2013 Jul 15;11:171. doi: 10.1186/1479-5876-11-171. A prospective, non-randomized, no placebo-controlled, phase Ib clinical trial to study the safety of the adipose derived stromal cells-stromal vascular fraction in idiopathic pulmonary fibrosis. Tzouvelekis A1, Paspaliaris V, Koliakos G, Ntolios P, Bouros E, Oikonomou A, Zissimopoulos A, Boussios N, Dardzinski B, Gritzalis D,Antoniadis A, Froudarakis M, Kolios G, Bouros D.
14. Iran Red Crescent Med J. 2013 Mar;15(3):256-9. doi: 10.5812/ircmj.7975. Lateral Traumatic Esophago-Cutaneous fistula in a Child; Platelet-Rich Fibrin Glue Challenge. Joudi M, Hamidi Alamdari D, Hyradfar M, Rahimi HR, Saremi E, Fathi M, Shojaeian R, Koliakos G.
15. Cell Tissue Bank. 2014, 15(3):491-9. A simple method for the quantitation of the stem cells derived from human exfoliated deciduous teeth using a luminescent cell viability assay.
Tsagias N, Koliakos KK, Spyridopoulos T, Pitiakoudis M, Koliakos E, Korpeti I, Koliakos G.
16. Histol Histopathol. 2014 Nov 3. [Epub ahead of print] Pluripotent stem cells isolated from umbilical cord form embryonic like bodies in a mesenchymal layer culture. Kouzi-Koliakos K, Karagiannis V, Tsikouras P, Koliakos GG.
Spyridopoulos T, Lambropoulou M, Pagonopoulou O, Birbilis T, Tsaroucha AK, Kouzi-Koliakou K, Botaitis S, Deftereou TE, Gaitanidis A, Pitiakoudis M.
J Reconstr Microsurg. 2015 Sep;31(7):516-26. doi: 10.1055/s-0035-1555751. Epub 2015 Jun 30.
18. Front Neurosci. 2015 Jul 14;9:247. doi: 10.3389/fnins.2015.00247. eCollection 2015. Stem cell models of polyglutamine diseases and their use in cell-based therapies. Siska EK1, Koliakos G2, Petrakis S
19. Graefes Arch Clin Exp Ophthalmol. 2015 Jul;253(7):1121-35. doi: 10.1007/s00417-015-3042-y. Epub 2015 May 23.Mesenchymal stem cells improve healing of the cornea after alkali injury. Almaliotis D, Koliakos G, Papakonstantinou E, Komnenou A, Thomas A, Petrakis S, Nakos I, Gounari E, Karampatakis V. 1Laboratory of Experimental Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece,
20. Gene Ther. 2014 Feb;21(2):158-67. doi: 10.1038/gt.2013.68. Epub 2013 Nov 28. Intratumoral gene therapy versus intravenous gene therapy for distant metastasis control with 2-diethylaminoethyl-dextran methyl methacrylate copolymer non-viral vector-p53.
Baliaka A, Zarogoulidis P, Domvri K, Hohenforst-Schmidt W, Sakkas A, Huang H, Le Pivert P, Koliakos G, Koliakou E, Kouzi-Koliakos K, Tsakiridis K, Chioti A, Siotou E, Cheva A, Zarogoulidis K, Sakkas L
21. Iran Red Crescent Med J. 2013 Mar;15(3):256-9. doi: 10.5812/ircmj.7975. Epub 2013 Lateral Traumatic Esophago-Cutaneous fistula in a Child; Platelet-Rich Fibrin Glue Challenge. Joudi M, Hamidi Alamdari D, Hyradfar M, Rahimi HR, Saremi E, Fathi M, Shojaeian R, Koliakos G.
22. Diagn Pathol. 2014 Nov 14;9:195. doi: 10.1186/s13000-014-0195-7. A gene therapy induced emphysema model and the protective role of stem cells. Zarogoulidis P, Hohenforst-Schmidt W, Huang H, Sahpatzidou D, Freitag L, Sakkas L, Rapti A, Kioumis I, Pitsiou G, Kouzi-Koliakos K, Papamichail A, Papaiwannou A, Tsiouda T, Tsakiridis K, Porpodis K, Lampaki S, Organtzis J, Gschwendtner A, Zarogoulidis K.
PARTICIPATION IN CONGRESSES
1. Kouzi-Koliakos K.
“Collection, processing, quality control and cryopreservation of umbilical cord blood stem cells.”
European Society of Gynaecological Oncology. Perspectives in gynaecological oncology & colposcopy teaching session. Skopia, September 28-30, 2007
2. Tsagias Ν., Κouzi-Koliakou Κ., Κaragiannis V., Ιoannidou Ε., Koliakos G.
“Isolation, cryopreservation and culture of stem cells from the umbilical cord blood”
21st Northern Greek Medical Congress, Thessaloniki 2007.
3. Tsagias Ν., Kouzi-Koliakou Κ., Karagiannis V., Ioannidou Ε., Koliakos G.
“Isolation and proliferation of μονοκυτταρικού population, from adult’s peripheral blood.”
21st Northern Greek Medical Congress 2007.
4. Tsagias Ν., Kouzi-Koliakou Κ., Κaragiannis V., Ioannidou Ε., Koliakos G.
“Isolation, culture and cryopreservation of stem cells from umbilical cord blood and adult’s peripheral blood”
28thBiology Congress Kavala 2007
5. Tsagias Ν. Hamidi-Alamdari D., Kouzi-Koliakou Κ., Michaloudi Ε., Karagiannis V., Koliakos G.
“High recovery of stem cells from the umbilical cord blood using a triple collection bag.”
19thPan-Hellenic Hematologic Congress 2007
6. Daniilidis Α., Klearchou Ν., Kouzi-Koliakou Κ., Tsagias Ν., Mauromichali Μ., Karagiannis V.
“Tracing of foetal erythrocytes in the circulation of pregnant women”
4th Pan-Hellenic Congress of the Greek Embryology Medicine, Kamena Vourla 2006
7. Kouzi-Koliakou Κ.
“Present and future applications of the stem cells from the umbilical cord blood”
10th Pan-Hellenic Congress. The Pan-Hellenic Congress. The development of obstetrics science, Patra 2006.
8. Tsagias Ν., Hamidi Alamdari D., Kouzi-Koliakou Κ., Koliakos G.
“Isolation and identification of the pluripotent population of stem cells from placenta.”
Award Best Study in Applied Research. 33rd Pan-Hellenic Medical Congress Athens 2007.
9. Antonitsis P., Ioannidou Ε., Kaidoglou Α., Kouzi-Koliakou Κ., Kalogeridis Α., Klonizakis Ι., Moros Ι., Papakonstantinou Ch.
“In vitro study for the transformation of the primitive mesenchymal cells from the bone marrow into myocardial cells.”
Award 22nd Pan-Hellenic Medical Congress Thessaloniki 2007.
10. Kouzi-Koliakou Κ.
“Present and future applications of stem cells from the umbilical cord blood”
Obstetrics and Gynecological Clinic, University of Ioanninon, Medical School. Continuous training programme for doctors, 2006-2007.
11. Tsagias Ν., Kouzi-Koliakou K., Β. Karagiannis V., Koliakos G.
“New method to collect increased amount of primitive hematopoietic cells from the umbilical cord-placenta, sufficient for adult transplantation”
15th Pan-Hellenic Transplantation Congress Thessaloniki 2007, Award by the Greek Transplantation Organisation.
12. Kouzi-Koliakou K.
“Present and future application of stem cells”
Round Table 5th Congress Εμβρυομητρικής Medicine Volos, 19/5/2008.
13. Kouzi-Koliakou K., Koliakos G., Karakitsos G., Karanastasi S.
“Mesenchymal stem cells from deciduous teeth and third molars.”
38th Pan-Hellenic Dental Congress Patra 2008
PATENTS GRANTED TO BIOHELLENIKA
1. Patent Diploma Number 1007478 with all the legal attached relevant documents certified: KOLIAKOS GEORGE
Biohellenika, Georgikis Scholis 65, 57001, Thermi (Thessaloniki)
Title: Method of stem cells collection from the rich in hematopoietic and mesenchymal cells placenta blood.
2. Patent Diploma Number 1007483 with all the legal attached relevant documents certified: KOLIAKOS GEORGE
Biohellenika, Georgikis Scholis 65, 57001, Thermi (Thessaloniki)
Title: System of preservation of biological samples in cryovials integrally attached.
3. Patent Diploma Number 1007490 with all the legal attached relevant documents certified: KOLIAKOS GEORGE
Biohellenika, Georgikis Scholis 65, 57001, Thermi (Thessaloniki)
Title: Method of mesenchymal stem cell isolation from the total length of the
umbilical cord.
4. Patent Diploma Number 1005560 with all the legal attached relevant documents certified: KOLIAKOS GEORGE, DARYOUSH HAMIDI-ALAMDARI
Biohellenika, Georgikis Scholis 65, 57001, Thermi (Thessaloniki).
Title: Measurment of oxidant-antioxidant balance in fluids.
5. Patent Diploma Number 1007477 with all the legal attached relevant documents certified: KOLIAKOS GEORGE, DARYOUSH HAMIDI,MOZAFARI MOSANEN
Biohellenika, Georgikis Scholis 65, 57001, Thermi (Thessaloniki).
Title: Measurment of oxidant-antioxidant balance in fluids.
K KOUZI-KOLIAKOS
Professor of Medicine
Medical Director of Biohellenika S.A.